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anti p enos  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti p enos
    Anti P Enos, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+enos/Phospho-eNOS+(Ser1177)+Rabbit+mAb/pmc13022698-99-18-20
    Average 95 stars, based on 340 article reviews
    anti p enos - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Common and distinct effects of sodium-glucose cotransporter-2 inhibitors on in vitro platelet activation.
    Article Snippet: Recent studies have demonstrated antiplatelet activity of sodium–glucose cotransporter-2 (SGLT2) inhibitors, particularly at high micromolar concentrations (10–30 μM).. However, the effects of clinically relevant nanomolar concentrations on platelet function have not been reported.. This study investigated the roles of empagliflozin and dapagliflozin, primarily at 0.625 μM, on platelet functions.

    Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets
    Article Snippet: Protein levels were quantified with NIH ImageJ software. .. Antibodies were diluted in the blocking solution and used as follows and are listed in Supplementary Table : anti-IRE1α, 1:1000 (CST, 3294); anti-XBP1, 1:1000 (ABcam, ab220783); anti-Flag, 1:2000 (ABclonal, AE005); anti-Myc, 1:2000 (ABclonal, AE010); anti-β-Actin, 1:50000 (ABclonal, AC026); anti-phospho-IRE1α-S724, 1:1000 (ABclonal, AP1146); anti-Akt, 1:2000 (CST, 9272); anti-phospho-Akt-S473, 1:2000 (CST, 4060); anti-TSP1, 1:1000 (CST, 37879); anti-eNOS, 1:1000 (CST, 32027); anti-phospho-eNOS-S1177, 1:1000 (CST, 9570) and anti-α-Tubulin, 1:5000 (Sigma-Aldrich, T6199). .. Total RNA was extracted from isolated islets of HFD-fed Ern1 fl/fl and Ern1 EC-KO mice and then subjected to commercial RNA-seq analysis (Beijing Genomics Institute).

    Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets.
    Article Snippet: Protein levels were quantified with NIH ImageJ software. .. Antibodies were diluted in the blocking solution and used as follows and are listed in Supplementary Table 4: anti-IRE1α, 1:1000 (CST, 3294); anti-XBP1, 1:1000 (ABcam, ab220783); anti-Flag, 1:2000 (ABclonal, AE005); anti-Myc, 1:2000 (ABclonal, AE010); anti-β-Actin, 1:50000 (ABclonal, AC026); anti-phospho-IRE1αS724, 1:1000 (ABclonal, AP1146); anti-Akt, 1:2000 (CST, 9272); antiphospho-Akt-S473, 1:2000 (CST, 4060); anti-TSP1, 1:1000 (CST, 37879); anti-eNOS, 1:1000 (CST, 32027); anti-phospho-eNOS-S1177, 1:1000 (CST, 9570) and anti-α-Tubulin, 1:5000 (Sigma-Aldrich, T6199). .. Total RNA was extracted from isolated islets of HFD-fed Ern1fl/fl and Ern1EC-KO mice and then subjected to commercial RNA-seq analysis (Beijing Genomics Institute).

    Membrane:

    Article Title: Common and distinct effects of sodium-glucose cotransporter-2 inhibitors on in vitro platelet activation.
    Article Snippet: Recent studies have demonstrated antiplatelet activity of sodium–glucose cotransporter-2 (SGLT2) inhibitors, particularly at high micromolar concentrations (10–30 μM).. However, the effects of clinically relevant nanomolar concentrations on platelet function have not been reported.. This study investigated the roles of empagliflozin and dapagliflozin, primarily at 0.625 μM, on platelet functions.

    Incubation:

    Article Title: Common and distinct effects of sodium-glucose cotransporter-2 inhibitors on in vitro platelet activation.
    Article Snippet: Recent studies have demonstrated antiplatelet activity of sodium–glucose cotransporter-2 (SGLT2) inhibitors, particularly at high micromolar concentrations (10–30 μM).. However, the effects of clinically relevant nanomolar concentrations on platelet function have not been reported.. This study investigated the roles of empagliflozin and dapagliflozin, primarily at 0.625 μM, on platelet functions.

    Article Title: Sodium nitroprusside improves ischemia-induced neovascularization via the HO-1/NOX and AKT/eNOS signaling pathways in chronic kidney disease.
    Article Snippet: Aims: In chronic kidney disease (CKD), the incidence of peripheral arterial disease is elevated and is a major source of morbidity and mortality.. Accumulated circulating uremic toxins, particularly asymmetric dimethylarginine (ADMA) and indoxyl sulfate (IS), are shown to increase oxidative stress and reduce nitric oxide bioavailability and generation, ultimately leading to vascular endothelial cell damage.. We tested the hypothesis that sodium nitroprusside (SNP) could enhance ischemia-induced neovasculogenesis in CKD and investigated the mechanism behind this effect.

    Western Blot:

    Article Title: O-GlcNAcylation of eNOS in high-salt-induced thoracic aorta endothelial dysfunction in mice
    Article Snippet: Protein bands were visualized using enhanced chemiluminescence (ECL) reagent, and images were acquired with a Bio-Rad imaging system (Bio-Rad Laboratories, United States). .. The primary antibodies used for Western blotting were: anti-eNOS (Cat. #32027, Cell Signaling Technology, United States), anti-O-GlcNAc (Cat. #05-1245, Sigma, United States), anti-OGT (Cat. #ab177941, Abcam, England), anti-OGA (Cat. #ab124807, Abcam, England), anti-eNOS (Cat. #32027, Cell Signaling Technology, United States), anti-p-eNOS Ser1177 (Cat. #9571, Cell Signaling Technology, United States), anti-Caveolin-1 (Cat. #16447-1-AP, Proteintech, China) and anti-GAPDH (Cat. #60004-1, Proteintech, China). ..



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    MedChemExpress vascular endothelial growth factor vegf protein
    Schematic illustration of the a) preparation and application of Ti-OH-ePV; b) <t>VEGF</t> release of Ti-OH-ePV under different pH conditions; c) anastomotic healing performance with Ti and Ti-OH-ePV; d) healing-promotion mechanism of Ti-OH-ePV.
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    Cell Signaling Technology Inc anti p enos
    Schematic illustration of the a) preparation and application of Ti-OH-ePV; b) <t>VEGF</t> release of Ti-OH-ePV under different pH conditions; c) anastomotic healing performance with Ti and Ti-OH-ePV; d) healing-promotion mechanism of Ti-OH-ePV.
    Anti P Enos, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic illustration of the a) preparation and application of Ti-OH-ePV; b) VEGF release of Ti-OH-ePV under different pH conditions; c) anastomotic healing performance with Ti and Ti-OH-ePV; d) healing-promotion mechanism of Ti-OH-ePV.

    Journal: Bioactive Materials

    Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

    doi: 10.1016/j.bioactmat.2026.01.005

    Figure Lengend Snippet: Schematic illustration of the a) preparation and application of Ti-OH-ePV; b) VEGF release of Ti-OH-ePV under different pH conditions; c) anastomotic healing performance with Ti and Ti-OH-ePV; d) healing-promotion mechanism of Ti-OH-ePV.

    Article Snippet: Vascular Endothelial Growth Factor (VEGF) protein was supplied by MedChemExpress LLC (USA).

    Techniques:

    Structure characterization of Ti-OH-ePV. a) SEM images of Ti, Ti-OH, and Ti-OH-ePV (scale bars: 1 μm); b) Elemental mapping of Ti-OH-ePV; c) CV test of DA and VEGF solution under a nitrogen atmosphere; d) AFM height images of Ti, Ti-OH, and Ti-OH-ePV; e) Surface Sa (arithmetic mean height) via AFM of Ti, Ti-OH, and Ti-OH-ePV; f) Water contact angle of Ti, Ti-OH, and Ti-OH-ePV; g) FTIR spectra of Ti, Ti-OH, and Ti-OH-ePV; h) Tensile testing of the Ti, Ti-OH, and Ti-OH-ePV; i) Single anastomotic staple tensile strength testing of the Ti, Ti-OH, and Ti-OH-ePV; j) VEGF release profiles of the Ti-OH-ePV, Ti-ePV, and Ti-OH-PV in buffer solutions at pH = 7.4; k) VEGF release profiles of the Ti-OH-ePV, Ti-ePV, and Ti-OH-PV in buffer solutions at pH = 6.5; n = 3; ns = not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Bioactive Materials

    Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

    doi: 10.1016/j.bioactmat.2026.01.005

    Figure Lengend Snippet: Structure characterization of Ti-OH-ePV. a) SEM images of Ti, Ti-OH, and Ti-OH-ePV (scale bars: 1 μm); b) Elemental mapping of Ti-OH-ePV; c) CV test of DA and VEGF solution under a nitrogen atmosphere; d) AFM height images of Ti, Ti-OH, and Ti-OH-ePV; e) Surface Sa (arithmetic mean height) via AFM of Ti, Ti-OH, and Ti-OH-ePV; f) Water contact angle of Ti, Ti-OH, and Ti-OH-ePV; g) FTIR spectra of Ti, Ti-OH, and Ti-OH-ePV; h) Tensile testing of the Ti, Ti-OH, and Ti-OH-ePV; i) Single anastomotic staple tensile strength testing of the Ti, Ti-OH, and Ti-OH-ePV; j) VEGF release profiles of the Ti-OH-ePV, Ti-ePV, and Ti-OH-PV in buffer solutions at pH = 7.4; k) VEGF release profiles of the Ti-OH-ePV, Ti-ePV, and Ti-OH-PV in buffer solutions at pH = 6.5; n = 3; ns = not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: Vascular Endothelial Growth Factor (VEGF) protein was supplied by MedChemExpress LLC (USA).

    Techniques:

    a) The procedure of gastrointestinal anastomosis in New Zealand rabbit; b) Immumohistochemical staining images of IL-6, TNF-α, TGF-β, and IL-10 at the anastomotic stoma on day 3 for Ti, Ti-OH, Ti-OH-ePDA (PDA-only), and Ti-OH-ePV (VEGF-loaded PDA) groups (scale bar: 25 μm); c) Statistical analysis of IL-6 expression in different groups; d) Statistical analysis of TNF-α expression in different groups; e) Statistical analysis of TGF-β expression in different groups; f) Statistical analysis of IL-10 expression in different groups; n = 3; ns = not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Bioactive Materials

    Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

    doi: 10.1016/j.bioactmat.2026.01.005

    Figure Lengend Snippet: a) The procedure of gastrointestinal anastomosis in New Zealand rabbit; b) Immumohistochemical staining images of IL-6, TNF-α, TGF-β, and IL-10 at the anastomotic stoma on day 3 for Ti, Ti-OH, Ti-OH-ePDA (PDA-only), and Ti-OH-ePV (VEGF-loaded PDA) groups (scale bar: 25 μm); c) Statistical analysis of IL-6 expression in different groups; d) Statistical analysis of TNF-α expression in different groups; e) Statistical analysis of TGF-β expression in different groups; f) Statistical analysis of IL-10 expression in different groups; n = 3; ns = not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: Vascular Endothelial Growth Factor (VEGF) protein was supplied by MedChemExpress LLC (USA).

    Techniques: Staining, Expressing

    a) Immumohistochemical staining images of CD31 on day 7 at the anastomotic stoma for Ti, Ti-OH, Ti-OH-ePDA (PDA-only), and Ti-OH-ePV (VEGF-loaded PDA) groups (scale bar: 25 μm); b) Masson staining images on day 14 at the anastomotic stoma for Ti, Ti-OH, Ti-OH-ePDA (PDA-only), and Ti-OH-ePV (VEGF-loaded PDA) groups (scale bar: 20 μm); c) H&E staining images on day 14 at the anastomotic stoma for Ti, Ti-OH, Ti-OH-ePDA (PDA-only), and Ti-OH-ePV (VEGF-loaded PDA) groups; d) Statistical analysis for the number of blood vessels in different groups; e) Statistical analysis of collagen expression in different groups; f) Statistical analysis of bursting pressure on days 7, and 14 in different groups; g) Statistical analysis of WBC on days pre-1, 3, 7, and 14 in different groups; h) Statistical analysis of APTT on days pre-1, 3, 7, and 14 in different groups; i) Statistical analysis of ALT on days pre-1, 3, 7, and 14 in different groups; n = 3; ns = not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Bioactive Materials

    Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

    doi: 10.1016/j.bioactmat.2026.01.005

    Figure Lengend Snippet: a) Immumohistochemical staining images of CD31 on day 7 at the anastomotic stoma for Ti, Ti-OH, Ti-OH-ePDA (PDA-only), and Ti-OH-ePV (VEGF-loaded PDA) groups (scale bar: 25 μm); b) Masson staining images on day 14 at the anastomotic stoma for Ti, Ti-OH, Ti-OH-ePDA (PDA-only), and Ti-OH-ePV (VEGF-loaded PDA) groups (scale bar: 20 μm); c) H&E staining images on day 14 at the anastomotic stoma for Ti, Ti-OH, Ti-OH-ePDA (PDA-only), and Ti-OH-ePV (VEGF-loaded PDA) groups; d) Statistical analysis for the number of blood vessels in different groups; e) Statistical analysis of collagen expression in different groups; f) Statistical analysis of bursting pressure on days 7, and 14 in different groups; g) Statistical analysis of WBC on days pre-1, 3, 7, and 14 in different groups; h) Statistical analysis of APTT on days pre-1, 3, 7, and 14 in different groups; i) Statistical analysis of ALT on days pre-1, 3, 7, and 14 in different groups; n = 3; ns = not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: Vascular Endothelial Growth Factor (VEGF) protein was supplied by MedChemExpress LLC (USA).

    Techniques: Staining, Expressing